Coding

Part:BBa_K422009

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-25)

tetR (AarI A-part)

Biological Background

The tetracyclin repressor tetR binds to its operator tetO on the DNA and can influence gene expression or spatially localize a fusion protein to the DNA.

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. TetR-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 22
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None